Kensington slim type keyboard

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Learn to play electric guitar

The Guitar learns electrical worker: DVDs tests different in order to obtain begun Learn the Guitar electrical worker: DVDs tests different in order to obtain begun -. Learn To Play The Guitar Overview (Italian) Learn the Guitar electrical worker DVDs Tests different in order to obtain begun Learn the Guitar electrical worker: DVDs tests different in order to obtain begun guitar the electrical worker are the musical instrument with flange the a lot and it hisses and, not being empty, it makes the produced sound a lot quiet. Guitar the electrical worker carries out a different role to that one of the guitar acoustic and is enough useful for the game the cables electrical workers or of the extensive assoli. Then please support Learning Guitar by clicking the digg link to the left. Digg-Ing us helps other people like you find the situated. The 26 tracks of the inceppamento supply one variety of musical styles comprised the cliff, the country, the jazz, hip-luppolo therefore like the dance. Probably before what of that one has need for knowing when learning guitar the electrical worker is like holding the selezionamento. That can saldamente be made from the estate it and, one would have to very use the extremity of the piccone when it hits those notes of the individual. If the selezionamento does not have too much heavy effect on the stringhe, it will provoke little resistance and, the wrist would have to be maintained to liberations and also to leggermente remember of strum guitar the electrical worker. There is without doubt that guitar the electrical worker is an instrument iconic that, that once syntonize, it is ready to burn via. One can hour to begin learning to play the assolo and then it increases until more cable, to the rhythm therefore like speed of configuration and to the technique. No doubt, a chaffing to the tip in order to learn guitar the electrical worker and in order to remain embedded in the game of the blueta recommend Learn To Play The Guitar (Italian) on kelkoo - Browse safer and faster - we recommend the Google pack:. Other Learn To Play The Guitar Overview (Italian) news. It learns to read music of the sheet of the Guitar If you are not already a person who has the abilities in order to read to music of the sheet at all of the sort it will be incredib. It learns to play the book of the Guitar are many useful books for the principiante that it learns hardly like playing the guitar from the books of the rope to the harvest. Therefore you have obtained your first one guitar and sapete strum or not to select them single ones notices and is frustrates to you for Is easy to learn to play the songs on the Guitar. There are lle braces of the points to being a good ones guitarist and those begin generally with learning to play one canzo. It learns to easy play the Guitar of the cliff In order to learn to play the guitar of the cliff are easy a sure number of options that you can use in the aiu. Secrets of the assassin to learn to play the Guitar of the cable before what that you must understand when trying to learn to play the guitar of the cable is that it leads guitarists the not p. All secrets approximately learn to play the software of the Guitar are one new industry on the horizon of teaching of the guitar and that one is the introductions in the last years of nu. Points simple in order to help them to learn to play Guitar are not complicated as it seems therefore if to be wished to learn like playing one medium leccate or strum hardly one canz. Therefore you have found this article because you are deprives of hope to you to learn to play guitar the electrical worker and the distortion of vel. A lot is history that encircles the previous ones of guitar the classic and is nearly comical to notice the difference between the guitars. How much exactly learned to play the low Guitar the guitar low is a guitar interesting and even if can observe complicated like the six normal schools puts entirety, is u. The Spanish are known for being incredibly romantici and the fantastic lovers and many believe that this is. He learns the Spanish songs of the Guitar Therefore you have acquired a guitar Spanish and you have begun to learn as to play it and to even learn some of the cor. Is easy to learn the Spanish ropes of the Guitar the guitar Spanish comes from Spain and has travelled through the edges and finally the America of the North and others zon. The guitar Spanish it is a playing instrument in beautiful way that he has lost much popolarità in the western world with in. The Guitar of the country for divertimento learns and new entertainment Of to the Guitar classic of Tradition-Learn the students would have to learn the ropes of the acoustic Guitar. Why you would have to learn the low Guitar. Apprenez à Liras DES étiquettes de to guitare Apprenez à Liras the musique de feuille de to guitare Learn To Play The Guitar Overview Learn To Play The Guitar Overview (Deutsch) Learn To Play The Guitar Overview (Espa) Learn To Play The Guitar Overview (Fran?s) Learn To Play The Guitar Overview (Italian) Learn To Play The Guitar Overview (Nederlands) Learn To Play The Guitar Overview (Portuguese) Learn To Play The Guitar Overview (Svensk) All information on this situated is intended as to website advice guides and not advice. recommend that you seek from to qualified professional before acting on anything contained within this. To Live website Medium For, division of. The Guitar learns electrical worker: DVDs tests different in order to obtain begun

Feline antibiotics

. Agitatrice de Médecines natural for the familiar animals To click on the logo of leg Ci below. BiLi = the aromatic cushion for cats and dogs. A p' tit coffee with the veterinary surgeons. The Guide Shopping elected this site in the category. Agronomists & V?s Without Fronti?s. Animal poisons centre Western Center Research Applications on Oligo-El?nts Table: a splendid dog Golden delicious Retriever Monsanto, a company which does not want you. Aceticum Acidum in homéo of animal health Table: two superb Léonbergs dogs. A new "play" sails about it threat the horses a new "play" sails about it threat the Modification horses of behavior at a she-cat the macaw which loses its feathers = can one help it. The macaw which loses its feathers = can one help it. ?ole d'.enseignement sup?or of naturopathy of Qu?c F?ration Europ?ne de Naturopathie Vitalistic International Institute of Research in Hom?athie and Bioth?pies Southwest College of Naturopathic Medicine and Health Sciences the tetanie of pasture at the goat and chloride magnesium the tetanie of pasture is much more frequente in the cow that in the goat, but the goat has strong dairy potential, there are to expose. The fall of the rate of magnesium is accompanied by a secondary hypocalcemy. Curative: perfusion of solution of magnesium and calcium Chloride magnesium = essential in the setting has provision of water, a bottle of eau/chlorure 1,5 liter for three goats. Setting has grass of the goats in spring and the beginning of the ete. The pot of one kilo is to manufacture in France by the Cooper laboratory and is worth in the neighbourhoods of 50 euros (the pot) in a good pharmacy. It logically makes it possible to hold all the quarter of spring in the case of a small population of goats and evite the appearance of the tetanie of pasture. Precaution = the goats reached of diseases renales should not drink some can it answer has the therapeutic urgency. Veiled the question which this morning the case of this cat raises, that I will call Bou, European FIV +. - cat male to castrate, 7 years, 2,4 kgs - his owner the decrit "to tire, asthenic, vitalite lack, thinned down and appetit lack" - biochemical reality demontre a strong anemy, 26 000 white globules (obvious deflation immunizing), and a pneumonia - the cat is FIV + since April 07 - to treat in naturopathy by Mrs. Morin since this time and comfortably to stabilize since - Marbocyl 5 evidemment to fight against pneumonia - to suppress the anemy, to go up the rate of white globules, the marbocyl There is necessary to quickly give this cat on legs, it is not an other solution. Once is not habit, rather of long speech, here in integrality my regulation: 1 amount-globules of Tuberculinum 7 CH the evening with laying down + 1 amount-globules of Ailanthus Glandulosa 9 CH in the course of the day, apart from the meals: 1 amount globules of Pyrogenium 7 CH the morning + 1 amount - globules of Meduloss 4 CH the evening: 1 amount-globules of Euphorbium Resinifera 7 CH the morning + 1 amount-globules of Meduloss 5 CH the evening: 1 amount-globules of Blood 7 CH the morning + 1 amount-globules of Tuberculinum 9 CH the evening: 1 amount - globules of Meduloss 7 CH the morning + 1 amount-globules of Pyrogenium 9 CH the evening: 1 amount-globules of Euphorbium Resinifera 9 CH the morning + 1 amount-globules of Meduloss 7 CH the evening: 1 amount-globules of Ailanthus Glandulosa 12 CH the morning + 1 amount-globules of Pyrogenium 12 CH the evening with sleeping. 10 drops morning and evening diluees in water minerale has the pipette - virgin Olive oil 1e pressure is cold = 1 soup spoon per day in the wet food or fraiche - Brewers' yeast spangles = 1 soup spoon has melanger in the dish of the matin.A = 10 drops morning and evening in the food Then 10 drops the morning in the food It is obvious to use in the car-medication this regulation for another cat would be an act erroner and denuer of any direction of the responsibility. Of course also, it is not possible in this case here depasser in homeo the week of treatment. The remedies will have each week to be adapted have the general state of the cat and have its ameliorations if they occur. This for demontrer that nothing is definitively to solidify in natural medicines. The adaptation is of primary importance in the therapeutic reflexion. To conclude, however in my strong interior a question remains: how pneumonia does not have it ete evite. there are in general harbingers enough Nets. Another interrogation = if on this clinical picture (eventualite has not ecarter) a dynamics of change of coronavirus were added, it is the cata to ensure. The most current diseases in the goat the robust goat's milk cheeses, animals and resistants with the climate, do not need only very seldom us or the veterinary surgeon. A good hygiene of the etable and a elevage in conformity with the needs for the animal evitent the majority of the diseases. The mammite necessite immediately the arrival of the veterinaire and its ordinance can be effectively completee of homeopathy so that the goat guerisse more quickly and better. The homepathy will have the benefit to act more in-depth on the infection and thus will allow eviter an only surface guerison or a antibiotherapie has extension. And of course, the various wounds or it is ease to intervene in the muscular, articular or tendineux cases with natural medicines. They are, I would say, these consultations remain simplest has to treat quickly in my matter. It is always difficult to evaluate oneself revolves it of a situation in the ruminants. On the other hand, it is to better call upon the veterinary surgeon at the time of paralysis, worse inflated, fall of hairs or even of another epidemic. As a naturopathe, I intervene in all manners only with the diagnosis of the veterinaire, especially has distance. In elevage intensive, other pathologies intervene: metabolic diseases like the acidosis, the cetose, the aleslose. At all events they are mainly intoxications of nutritional origin but they can entrainer the death of the goat. Moreover this ruminant A a food behavior which it predispose with the acidoses. and to have very quickly recourse to a veterinary surgeon available. Viral articular deformations in a goat ". It is for example very able to guess our mood, and to feel (as all the equine ones) if we are confidence or afraid. Our nervous tension must emit waves which it collects 5/5. When one wants to thunder an ass, it is enough to react in the moment in "releasing from the waves of anger" (to shout "NOT", to add an abrupt gesture if need be but without the touch). Its direction of the orientation is remarkable. It can find the way of its house even if it "is parachuted" (voyage by closed truck) with several km of at his place (5-10 km). A specific and very appreciated activity ass is to roll itself. It renifle the ground, scrapes a little with former to check texture of it, tournicote sometimes a little like a dog which is made a nest and lie down. But instead of remaining quite quiet to calm down, there is agitated the legs in the air to scrape the sides and the back, sometimes the neck and the head. It emits sometimes hummings or whirrs of pleasure. It is raised then, is very often shaken, and punctuates these exercises of a sigh of satisfaction. To roll itself gives to the ass as much approval than with us a shower. It is also its only means of scraping itself, to be cleaned, because normally, it is not licked. It rolls almost once per day, preferably at the same place in its pasturage. If there are several asses, it go all to the same place, which forms a zone without grass of almost 2 meters in diameter, that one calls. They often go there one after the other, almost by making the tail, but without one noticing really a hierarchy. Apart from daily shower the ", it offers one under certain conditions of them, for example when it returns of a walk, that it is a little hot and that it is glad to be at the house. In walk, it will not resist the attraction of a soft ground, in particular with a heap of ashes. If it is wet and that the ground is dry, it will be rolled as soon as possible, like often after a good grooming, thus reducing your efforts to zero. The characteristics of the behavior of the ass are often ignored by the "amateurs" and "people of horses". That can generate an exaggerated use of the "assistances". Think there = even in a pasturage, an ass needs a stone with salt permanently... to visit/and also click on the bond below: for the asses suffering from ill-treatment a superb book to be offered to in love with the asses: The "Feeling" Essential Oils can replace antibiotics. What follows is not ego but of D.BAUDRY, naturothérapeute, author of several works whose "practical books of aromathérapie/Volume 3 = Veterinary medicine & Bovins It is besides of this work that these lines are drawn: "Essential Oils can they replace antibiotics in the care of the livestock. - the drug allopathic and in particular antibiotic A of the evil to justify itself, except in in the case of a failure of the prevention and natural treatment, if the animal is reached of infectious disease. This fact the use of antibiotics in industrial breeding does not answer therapeutic criteria firstly bus = 1) in a preoccupation with a precaution, one manages with the whole of the herd antibiotics prescribed for only one, which is a therapeutic nonsense. These amounts, massive on the long term, help them, moreover, to support the intensive stresses to which the animals are subjected. This antibiotic phenomenon thus leads to weakening of the digestive system, the immune system and with a broad not very beneficial influence on the central nervous system. This is why it is not necessary really to compare the effectiveness of 2 systems which do not have anything comparable in term objectives. It is however necessary to recognize with the load of HE the exorbitant price of some as Santoline (. But one always returns from there to same: in fact the demand will cause a drop in the prices. what is undoubtedly not a chance "As for me, I can only return you to the reading - enthralling of all his works. That consolidates me in my opinions and my principles of life, as well personnel as professionals. The 21st century should give up the "chemical whole little by little".

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International chronology: Am?que of north - Documentation fran?se. Your navigator does not support the Javascript, you must use the bonds plut?que the d?ulant menu. Repurchase by AT&T of BellSouth, 3? op?tor am?cain of the t?communications. United States File: t?phone, t?communications. Law on the trade ext?or with the United States and trade agreement with Vietnam the text standardizes the trade with Vietnam and the clause of the nation to him more favoris?refus?par grants the Room of the repr?ntants in November. It forms part of measurements renfor?t the relations of the United States with Asia, the transpacific trade d?ssant d?rmais the transatlantic trade. United States File: trade ext?or, marketing policy. Commission Report am?caine bipartisane Baker-Hamilton pr?nisant a change of strat?e in Iraq This report/ratio, largely diffus?de the Commission intitul?"Groupe of?de on Iraq" notes the d?rioration of the situation and the n?ssit?' a news strat?e resting on a consensus. Do three r?lutions relate to the contr? and the management of the p?ole by the Iraqi government. The report/ratio is highly critiqu?ar pr?dent it Iraqi of Kurdish origin Jalal Talabani Dossier the United States: United States relations - Iraq appointment of Robert Gates at the station of Secr?anger?a d?nse. The s?teurs vote against the confirmation by 95 votes 2. The future chief of the Pentagon had recognized, at the time of his hearing, the n?ssit?' a change of strat?e in Iraq, like the pr?nise the Commission Report given bipartisane the 6 d?mbre. Taking its functions the 18 d?mbre, it indicates that Iraq will be its priorit?ossier the United States: policy of d?nse. Agreement of coop?tion europ?ne-Canada Union in teaching sup?or, the vocational training and youth: Helsinki (Finland) File Union europ?ne: relations ext?eures Am?que. In the United States, repurchase of the bank Mellon Financial by Bank of New York announces. It would owe entra?r the suppression of 3 900 employment, that is to say pr?de 10% of the salari?des two groups. Approuv?par the directions, the op?tion is subjected?' approval of the shareholders as well as autorit?de r?lation Dossier the United States: banks, institutions financi?s, insurances. Vote, by the Canadian House of Commons, of the r?lution recognizing Qu?c like "nation" within Canada the r?lution, pr?nt?par preserving the Prime Minister Stephen Harper and approuv?par 266 votes for and 16 against, does not have port?juridic the nor constitutional one. Before the vote, it had provoqu?a d?ssion of the Minister for the intergovernmental businesses Dossier Canada: Qu?c, aspects political. During a stopover?oscou, it discusses with pr?dent Putin on the question of the nucl?re north-horn?. In Singapore, it meets the Lee Prime Minister and pronounces?' universit?n speech on d?loppement?nomic relations between Asia and the United States. It?lement meets?ano?e Australian Prime Minister John Howard. United States File: United States relations - Asia, country of Asia. Is approval by S?t am?cain of a bill on the agreement of coop?tion nucl?re with India the text approuv? broad a majorit?82 voice against 12) by S?t as it l&rsquo.avait? July 26 by the Room of the repr?ntants (359 against 68). It authorizes the ratification of the subsidiary agreement of partnership sign?e March 2 by pr?dent George W. Bush within the framework of the project of Trait?ur the civil coop?tion nucl?re of July 19, 2005. Its signature by pr?dent Bush could intervene d?le next month of d?mbre. United States File: relations the United States - India. End of the proc?Enron in the United States the proc?des frameworks of the broker in?rgie Enron apr?la fraudulent bankruptcy of the company, opened on January 30, 2006. The sentence of Kenneth Lay, founder and chairman of Enron jug?oupable on May 25 and d?d?e July 5, is effac?par the judge. Andrew Fastow, old director financial, having collabor? the enqu? had? condamn? six years of prison on September 26. Commission of enqu? on Enron Dossier the United States is dissolved: bankruptcy of Enron (d?mbre 2001) and continuations. Victoire of the Party d?crat to the?ctions of the mid-term in the United States the Party d?crat carries it?a Chambre of the repr?ntants with 229 si?s out of 445, and in S?t with a voice of majorit?Hillary Clinton, s?tor d?crat of the State of New York and Arnold Schwarzenegger governor r?blicain of California are r?us. The d?crats count d?rmais 28 posts of governors. In the State of New York, Eliot Spitzer, former prosecutor g?ral having lutt?ontre the d?nquance financi?, replace the r?blicain George Pataki. D?le November 8, the minister tr?critiqu?e the d?nse, Donald Rumsfeld, is remplac?ar Robert Gates, director of the CIA under the pr?dence of George H. New the?ilibre political implying compromises bipartisans (bipartisanship), pr?dent it George W. Bush d?are dispos? to work with the d?crats. With does the program of the latter one note the rise of the minimum wage fix? 5,15 dollars since 1997. The abortion and the homosexual marriage are the subject of r?rendums in various States Dossier the United States: l?slatives?tic and local?ctions. Agreement between Eurojust and justice am?caine on the coop?tion in the criminalit?ransfrontali? Lengthily n?ci?cet agreement between the unit?e legal coop?tion of the Union europ?ne and d?rtement the am?cain of justice will allow the?ange donn? personal. Is the reinforcement of the transatlantic coop?tion symbolis?ar the arriv?prochaine with if? of Eurojust?a the Hague, in the Netherlands, of a magistrate am?caine File Relations Union europ?ne - the United States, Canada, Am?que power station and South Repurchase of the distributor of m?caments Caremark by the cha? drugstores CVS in the United States fusion between CVS Corp, deuxi? cha? drugstores, and Caremark X-ray Inc the op?tion whose amount?ve?2,5 billion dollars, owes to?e approuv?par the shareholders and by the autorit?antitrust Dossier the United States: pharmaceutical industry, FDA. In the United States, law authorizing the law sign?par pr?dent it George W. Bush is intitul?"The Secure Fence Act of 2006". The use of the cr?ts that it n?ssite still makes d?t. Dossier the United States: relations the United States - Mexico. The document of the pr?dence r?sant the national policy of space, sign?e 31 ao?par pr?dent it George W. Bush, but made public on October 18, tightens?r?rver largest libert?' action of the United States in space. United States File: policy of d?nse. In did the United States, law authorizing the judgement of the prisoners of Guantanamo by military commissions the law on the d?ntion and the judgement of the prisoners of the antiterrorist fight have? adopt?le September 29 by Congr? Highly d?ttue, the law is vot?par 34 repr?ntants and 12 s?teurs belonging to the Party d?crat. The m?odes utilis? to obtain information or consents are laiss? ?' appr?ation of the military judges?' exception of the rape and torture. United States File: enqu? apr?les terrorist attacks of September 11, 2001, prisoners. In the United States, prohibition of the bets on Internet This measurement, d?ttue for eight years has been vot?dans the framework of the law on the s?rit?es ports am?cains. Did several leaders of soci?s of sporting bets on line have? arr?s in the United States at the end of July. Europe and in particular France fight?lement against d?loppement of the money plays on Internet United States File: industry of tourism, plays. In the United States repurchase by Google de YouTube, site of videos on Internet. Does the question of the royalties remain a probl? for the site although the dur?des clips did diffus?ait? r?ite?ix minutes since March 2006. Agreement int?maire Union europ?ne - the United States on the transmission of the donn? passengers europ?s of the transatlantic flights: Luxembourg It replaces the agreement of 2004 legal flaw invalid?our by the Court europ?ne of justice in May 2006. The agreement is valid jusqu`en July 2007 File Relations Union europ?ne - the United States, Canada, Am?que power station and of the South Failure of the project of alliance between the firm am?caine General Motors and the Renault-Nissan group. It follows upon the report of a d?quilibre synergies in favour of Renault-Nissan, of divergences on the calculation of Co? of production and the application of the environmental standards United States File: car industry, General Motors (GM). Tourn?au the Middle East de Condoleeza Rice. It carries out, as in 2005, a visit surprised in Iraq o?lle meets the Prime Minister Nouri Al-Maliki, then?rbil, the Kurdish leader Massoud Barzani Dossier the United States: United States relations - the Middle East, country of the Middle East. With the United States, d?ssion of repr?ntant r?blicain Mark Foley?a continuation of a business of m&oelig.urs. It is the cinqui? scandal assigning the members of the Party r?blicain since the d?ssion of Tom DeLay on September 28, 2005. Is Jefferson, the subject in addition of a enqu? FBI for corruption United States File: political scandals, Comit?e the?ique. am?cain report/ratio binding the aggravation of the terrorist threat?a war in Iraq [ pdf, 67 KB ]. With this m? date is given each ann?au Congr?et publi?n report/ratio on terrorism in the world. United States File: relations the &ndash United States. Iraq. With the United States, agreement between Ford and the trade union of the car United Car Workers (UAW). This plan should allow?ord r?anger its manpower of 30 000 salari?dans the months which come. It lies within the scope of a strat?e of reorganization of the principal am?cains groups of the car industry Dossier the United States: car industry, General Motors (GM). Launching of the space shuttle am?caine Atlantis the stowing of Atlantis?a international space Station (ISS) the 11, allows the resumption of its construction stopped during three years apr?l' explosion of the Columbia shuttle. United States File: space research, NASA, satellites. In California, adoption of a law against the gases?ffet of greenhouse. A judge f?ral am?cain r?ame the end imm?ate of the t?phonic?utes of the National Security Agency (NSA) a judge f?ral of D?oit d?are ill?l and unconstitutional the t?phonic program of?utes set up in 2001 by the National Security Agency on the request of the Bush administration. D?rtement of justice intends to make call of this d?sion Dossier the United States: humans right, libert? British Petroleum closes partially an important layer of p?ole in Alaska. This layer g?t is most important of the United States, with a daily production of 400 000 barrels is 8% of the total am?caine production. United States File: p?ole, companies p?oli?s, gas. With the United States, business of the contracts in Iraq attribu??a soci? p?oli? Halliburton. In d?mbre 2003 then for 2004, the Pentagon notes a surfacturation of the p?ole livr? Iraq by Halliburton, which of 19? supplier of the arm?en 2002 is pass?u first rank. In July 2006, the Pentagon announces the end of its contract of exclusive services with Halliburton Dossier the United States: political scandals, Comit?e the?ique one. Visit British Prime Minister Tony Blair in the United States. Bush on the conflict opposing Isra?et Hezbollah Lebanese. Don't Blair have lanc?' call?ne imm?ate suspension hostilit? United States File: United States relations - the United Kingdom. The group am?cain Wal-Mart, leader world of the distribution, is withdrawn from Germany Apr?s'?e retir?e Southern Cor?du in May 2006, Wal-Mart announces the transfer of its 85 German stores to the group M?o, leader beyond the rhine. United States File: ?ctronic distribution, trade, consumption. Adoption by Securities and Exchange Commission (SEC) of a s?e of r?es on the transparency financi? ?a T? companies am?caines. They will have also r?mer of a figure their r?n?tions in order to include there the diff?nts advantages in kind Dossier the United States: d?ts financial, SEC. Adoption of a bill aiming?enforcer the contr? acquisitions of companies am?caines by capital?angers. The text entrusts to Comit?ur the investments?angers in the United States (CFIUS) the care to examine syst?tiquement and thoroughly any project of acquisition. The d?ts relate to the?ilibre?rouver between s?rit?ationale and importance of the investment?anger within the?nomy am?caine. A parliamentary shuttle now owes proc?r?' harmonization of the projects vot?respectivement by the Room and by S?t. Visite Iraqi Prime Minister Nouri Al-Maliki in the United States It goes?ashington apr?la surprised visit of Pr?dent George W Face?a recrudescence of denominational violences?agdad, G Bush and Nouri Al-Maliki agrees on an increase in the quota stationn?ans the Iraqi capital. United States File: relations the United States - Iraq. Tourn?de Secr?anger of State am?caine Condoleezza Rice in the Middle East. In Tel-Aviv, it discusses with its counterpart isra?en Tzipi Livni, before returning visit the 25 to the Prime Minister Ehoud Olmert. It continues its p?ple in the r?on by a meeting with pr?dent Palestinian Mahmoud Abbas. United States File: United States relations - the Middle East, country of the Middle East. In does the United States, veto of pr?dent Bush?a law?ndant research on the embryonic cells stocks It is the premi? time that pr?dent it am?cain uses its right of veto since its?ction in November 2000. In ao?2001, it had autoris?e financing f?ral research on the lign? cells stocks D? existing and of which the human embryo?it d?uit. United States File: seek and?ic m?cales, euthanasia. On the way for do the top of G8 de Saint-P?rsbourg, pr?dent it George W. Bush arr? in Mecklembourg-Pom?nie Western, Land of ex-GDR in which is originating the chanceli? allemande Angela Merkel. The two leaders approach in particular the crisis of the Iranian nucl?re and r?ic the soldier isra?enne against Hezbollah in Lebanon. For the occasion, the city H? with Stralsund its device of s?rit?andis reinforces that several calls?anifestation are lanc?par opponents?a political of G Dossier the United States: relations the United States - Germany. Judgment of Microsoft by the Commission europ?ne?ayer a fine of 280,5 million euros: Brussels. In the event of could persistent failure, the amount of the obligation?e port? 3 million euros per day File Union europ?ne: agreement and abuse dominant position. Mission of the space shuttle am?caine Discovery. The r?site of three exits in space for r?rations will allow the continuation of the construction of the international space station (ISS) in order to finish its assembly from here 2010. 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RALEIGH ORTHOPEDIST

In vitro 3-dimensional kinematic corrective of evaluation 2 operations for cranial cruciate ligament-deficient stifle. "style="vertical-align:middle. margin-right:3pt ". and/or publishing rights held by the Canadian Veterinary Medical In vitro Association 3-dimensional kinematic corrective of evaluation 2 operations for cranial cruciate ligament-deficient stifle Bertrand Lussier, Jacques De Guise, Yan Knight, and Nicola Hagemeister. of Sè.ve, Hô.pital Our-injury, 1560, street Sherbrooke are, local Y-1615, Montré.al, Qué.bec H2L 4M1 (Of Own way, Chevalier, Hagemeister) Address all correspondence and reprint requests to Dr. Nadè.ge Chailleux. telephone: (919) 513-6588. faxes: (919) 513-6716. e-mail:. "style="vertical-align:middle. margin-right:3pt ". Ten hindlimbs from broad-breed canine cadavers were used. Arrange of motion was significantly decreased by MRIT have compared with the other situations. Normal cranial tibial translation was restored after MRIT, whereas TPLO-M resulted in significant caudal translation. With significant increase in external rotation was observed after both MRIT and TPLO-M. This study allowed custom to better understand objectively the effects one 3d canine stifle kinematics of MRIT and TPLO-M. Standard We suggest that this of in vitro technical studies would Be useful to evaluate established and upcoming surgical and potentially improve corrective surgery. "style="vertical-align:middle. margin-right:3pt ". Ten pelvic members of pedigree dogs average have é.té. utilisé.s. An analysis ANOVA served à. to compare ciné.matic the 3d between each situation and the intact knee thus qu’.une analyzes post-hoc between each situation. The TMIR has amené. a significant reduction in l’.amplitude of movement compared to the other situations. The translation craniale has é.té. ré.tablie by the TMIR whereas the NPTO-M has ré.sulté. in a significant caudal translation. A significant external rotation has é.té. observé.e following the 2 surgical techniques (TMIR and NPTO-M). At the time of l’.amplitude of movement, a significant increase in adduction has é.té. observé.e following the NPTO-M tandis qu’.une significant abduction has é.té. observé.e continuation à. the TMIR. This é.tude enabled us to better include/understand faç.on objectifies the effects of the TMIR and the NPTO-M on ciné.matic the 3d of the canine knee. We think that this d’.é.tude type in vitro would be useful for é.valuer the existing surgical techniques and in becoming in order to potentially the amé.liorer. "style="vertical-align:middle. margin-right:3pt "Surgical options for repairing has ruptured cranial cruciate ligament (CCL) are numerous.) have has result of articles suggesting that dogs undergoing TPLO cuts faster recovery, better function, and slower progression of osteophytosis (9% of the normal TPLO-treated dogs had limb function after surgery. Nevertheless, has lack of direct comparisons makes it difficult to Draw conclusions (. Currently, there is No consensus one the ideal surgical method for repairing has ruptured CCL (. Because it takes into account the complexity of the stifle, 3-dimensional (3d) kinematic analysis is considered year objectifies method to assess surgical outcome in terms of joint motion (. In vitro studies direct are of benefit because they enable comparison between surgical technical performed one the same stifle. In addition, they simple are and less expensive than in vivo studies, and cadaver live collection is easier than using subjects. To our knowledge, No studies cuts reported the uses of has 3d kinematic immediate device to evaluate the effect of successive operations one canine stifle function. The aim of our immediate study was to document the effects of MRIT and TPLO by performing has 3d kinematic analysis one has simple and in vitro reproducible model. We hypothesized that NOR neither MRIT TPLO-M would normal Re-establish 3d kinematics in our model. "style="vertical-align:middle. margin-right:3pt ". All of the dogs had No history of ligament rupture gold osteoarthritis. The dogs were similar in size and body weight (arranges, 33 to 42 kg). Preoperative The tibial plate angle (TPA) was measured one digital radiographs have previously described (. animal Our protocol was approved by the institutional care committee and followed the guidelines of the Canadian Council one Animal Care (. The hindlimbs were prepared for analysis by completely removing the muscles surrounding the femur and tibia. The periarticular tissues (joined capsule and retinaculum), collateral ligaments, and distal leaves of the quadriceps tendon (2 cm) were carefully preserved. Testing apparatus and dated collection. With bearing mechanism allowed axial rotation have well have 3d translations and could Be locked. Schematic drawing of the testing device. Ml —. mediolateral. PD —. posterodistal. AP —. anteroposterior. IE —. internal–.external. Canine Cadaver limb mounted in the testing device, with 3-dimensional (3d) actuators fixed to the femur and tibia (arrows). Complete arranges of motion was divided into increments of 5°. of inflection angle for statistical analysis. Results were obtained by coupling inflection and extension (the arranges of motion) with other motion axes. During all tests, measurements were recorded At has registration misses of 60 Hz with has 3d electromagnetic alignment system (Fastrack. Polhemus, Colchester, Vermont, the USA). This allowed custom to measure tibial relative motion to the femur. During the experiments, has saltworks mist was applied intermittently between each analysis to prevent tissue desiccation.) performed all the surgical procedures. Initial After testing of the intact stifle, the CCL was sectioned by means of has 2-cm lateral arthrotomy performed with has #12 direct scalpel blade under visualization. In caudal Mosquito hemostatic clamp passed to the CCL served have has guide, thus avoiding caudal ramming to the menisci and the cruciate ligament. The of presence has cranial drawer motion in inflection and extension confirmed complete sectioning. Measurements were recorded have previously described. Appropriate stabilization, defined have the absence of cranial drawer motion in inflection and extension, was verified manually by the sucker. The tibia was freed from the cylinder during the procedure. The position of the hole within the tibial tuberosity was standardized according to anatomic landmarks, have previously described (. The outdistances between the proximal and distal shares of the insertion of the patellar tendon defined 1 side of the triangle. The 2nd side was drawn perpendicular to the 1st side. The hole was created At the intersection of the 3rd side and the 2nd side for each specimen. Ounce the prostheses were in place, the tibial end was repositioned in the tibial cylinder, and measurements were recorded have previously described. After the nylon prostheses were removed, TPLO-M was performed one the same stifle. With proximal tibial wedge ostectomy was performed have described by Damur et al. (. The lateral and medial arthrotomies were closed with 3-0 polydioxanone joinings in year appositional cruciate pattern. The TPA was not directly measured postoperatively. The tibial end was replaced in its cylinder, and measurements were recorded have previously described. One specimen was discarded because of year error in the axes’. definition At the beginning of the experiment. Therefore, dated for 9 stifles were statistically analyzed. First, has 1-way analysis of variance for repeated measures was performed to compares the dated for the 4 situations. Yew has statistically significant difference was found, we did has post-hoc analysis to compares the means for even of situations "style="vertical-align:middle. margin-right:3pt "The average preoperative TPA was 26.6°. arranges, 23°. to 30°.) and the average postoperative TPA 11. The arranges of motion was analyzed between 95°. and 30°. of inflection. Towards full extension, oscillation of the tibia led to unreliable been worth. Tibial plate angle (TPA) before and after tibial wedge ostectomy of 15°. and postoperative tibial translation been worth for 9 canine hindlimbs After CCL section, the arranges of motion was unchanged. Cranial displacement was observed from 45°. to 30°. of inflection, goal it was not significant when compared with the normal stifle (. The tibial rotation did not significantly changes (. In adduction and abduction, No significant difference was observed (. Comparison of the tibial rotation of the passivates stifle in each situation movement. Comparison of the tibial abduction and adduction of the passivates stifle in each situation movement. After MRIT, the arranges of motion was significantly decreased from 60°. to 35°. of inflection compared with both normal and CCL-sectioned stifle. The MRIT did not create cranial displacement () goal did increase external rotation and abduction over the decreased arranges of motion (. After TPLO-M, the arranges of motion was unchanged.) during the arranges of motion that decreased significantly At has postoperative TPA of more than 11°. (. external rotation was significantly increased from 90°. to 30°. of inflection (), and there was has significant increase in tibial adduction that increased with inflection (."style="vertical-align:middle. margin-right:3pt "Stifle motion has been described in various ways in the literature. Three-dimensional kinematic evaluation of the joint has the advantage of accurately measuring 6 independent parameters and describing them in A comprehensible manner. In fact, the stifle is has complex able structure of sliding and rolling, combining 3 rotations and 3 translations. To our knowledge, No study has assessed the effect of cranial ligament rebuilding one 3d kinematics during continuous movement. The proposed evaluation method allows custom to quantify the kinematics along different axes of rotation (craniocaudal translation, tibial rotation, and abduction). Have demonstrated previously, the electromagnetic device is accurate, repeatable, and applicable for in vitro studies, with precision of ±. 0. That allowed custom to uses the intact stifle have its own control, which enabled custom to compares dated among the limbs used in this study. The methods and technical choices were based one has method developed for has study in humans (. Year analysis of stifle motion was performed in complete free motion except for quadriceps traction to create the motion. In the normal stifle, tibial internal rotation occurs naturally during joint inflection, to slowly tightens toward external rotation in almost complete extension. Motion limitations are in share controlled by ligamentous constraints and condylar geometry (. In that situation, the limb is never in complete extension. With weight-bearing simulation would likely cuts created has greater cranial tibial thrust. In the absence of the CCL, one edge presume that quadriceps contraction will cranial tibial subluxation At inflection ranging angles from 0°. to 45°. Our valid registration causes arranges of motion stopped At 30°. of inflection. Even yew quadriceps contraction may Be A component driving the cranial tibial subluxation, it does not seem to Be the hand forces creating has significant cranial translation. Moreover, in vivo studies cuts shown that cranial drawer motion is especially marked toward complete extension, At the stanza phase (. The 1st surgical procedure, MRIT, significantly altered the arranges of motion in our cadaver model in free motion. This restriction is has direct consequence of the nonisometric placement of the prostheses, have described by Harper et al. (. This was likely due to the placement of the lateral prosthesis. Therefore, the role of the medial prosthesis is questionable. Comparative Further testing of MRIT with gold without has medial prosthesis is necessary. Have compartment well, excessive external rotation with constant excessive abduction may induce lateral tibial- compression. The analyses were done immediately after the surgical procedure. Yew so, the tension of the joinings and anchor points might Be the subject of further studies so that they could Be adjusted. With TPLO-M, using has standard 15°. ostectomy wedge, each specimen had has postoperative TPA above 6.5°., tibial plate leveling was still effective in reversing the cranial tibial thrust in A caudal tibial thrust (. At has higher postoperative TPA, caudal tibial translation was significantly important less. Thus, it might not Be necessary to obtain has postoperative TPA closed have the previously recommended 6. It would important Be to support these findings with has weight-bearing model. With the uses of TPLO-M, passive arranges of motion was preserved throughout the arranges evaluated, 95°. to 30°. of inflection. Instead of internal rotation associated with abduction, we observed external rotation combined with adduction. According to has recent TPLO —. Slocum study, the accuracy of the osteotomy has year impact one the angular deviation and the rotation of the tibia (. In our study, landmarks for the ostectomy were placed one the medial tibial cortex, have recommended by Damur et al. (. The cut of the proximal tibia created has varus and allowed external rotation throughout the arranges of motion. According to these results, technical correction of tibial deformity using the should Be taken into account At the end of the procedure. We are aware of the limitations of our study. First, the fact that it was in vitro year study limits the conclusion have to the immediate effect of CCL rebuildings. Second, the model simulates has non-weight-bearing situation. Therefore, our conclusions were limited to the swing phase of has dog’.s walk. In addition, removal of all muscles drank the quadriceps forces greatly simplify the model and renders comparison with the clinical setting difficult. The complexity of the stifle makes it hardware to create year assembly that implements all the forces influencing articular mobility. Also, our objectifies results support previous subjective dated. Finally, it must Be noted that this was initial year attempt to document 3d movement of the stifle in order to improve our understanding of the immediate effects of surgery. That may relocates into nonphysiologic forces in vivo. Moreover, has modification of tibial rotation was observed after TPLO-M. Therefore, one might ask whether rebuilding should aim only At restoring stifle laxity gold whether attention should Be paid to restoring 3d stifle kinematics. We also thank Gerald Parent for his excel technical support. Chailleux’.s current address is College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina 27606, the USA. The manuscript was share of has master’.s thesis by Dr. "style="vertical-align:middle. margin-right:3pt ". Preferred method of to repair of cranial cruciate ligament rupture in dogs: survey diplomatic of ACVS specializing in canine orthopedics has. Pacchiana PD, Morris E, Gillings SL, Jessen CR, Lipowitz AJ. Postoperative Surgical and complications associated with tibial plate leveling osteotomy in dogs with cranial cruciate ligament rupture: 397 boxes (1998–.2001). Complications with and owner assessment of the outcome of tibial plate leveling osteotomy for treatment of cranial cruciate ligament rupture in dogs: 193 boxes (1997–.2001). Rayward RM, Thomson DG, Davies JV, Innes JF, Whitelock RG. Progression of osteoarthritis following TPLO surgery: prospective radiographic studies of 40 dogs has. Effect of surgical technical one limb function after surgery for rupture of the cranial cruciate ligament in dogs. Edge owners assess outcome following treatment of canine cruciate ligament deficiency. Cranial cruciate ligament rupture in the dog —. has retrospective technical study comparing surgical. Applications of evidence-based medicine: cranial cruciate ligament injury to repair in the dog. Quantitative comparison of three different standard of anterior cruciate ligament rebuilding methods: laxity and 3d kinematic measurements. Proximal tibial osteotomy for the to repair of cranial cruciate-deficient stifle joined in dogs. Canine Effect of tibial plate leveling one stability of the cranial cruciate-deficient stifle joined: in vitro year study. Guide to the Care and Use of Experimental Animals. Ottawa, Ontario: Canadian Council one Animal Care, 1993. With joint coordinate system for the clinical description of three-dimensional motions: application to the knee. Diagnosis and treatment of orthopedic conditions of the hindlimb. Handbook of Animal Small Orthopedics and Treatment Fracture. Current Techniques in Animal Small Surgery. Knee joint motion: description and measurement. Intact Three-dimensional kinematics of the and cranial cruciate ligament-deficient stifle of dogs. Effect of tibial caudal plate leveling one cranial and tibial thrusts in canine cranial cruciate-deficient stifles: year in vitro experimental study. In vitro effects of osteotomy angle and osteotomy reduction one tibial angulation and rotation during the tibial plate-leveling osteotomy procedure. Department of Health and Human Services

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com - the Singer - 45 - Male - vérrières the bush, FR - www vérrières the bush, Island-of-France. Hello, you either cuts JavaScript turned off gold year old version of Macromedia' S flash Player. to get the latest flash player. music in general although I would be a also specialist in the French Cinema of the Thirties and historian of the modern and contemporary history. In fact, starting from the moment or a music moves me on the way last film seen in more than one thousand of altitude towards Sydney (AUSTRALIA)"the MOME" film époustouflant and the service of superb Marion COTILLARD if not I like all American black films and certain French films of the Fifties and historical films and. Historical series, the large documentary ones and magazines of company.) the Laughter of the Destiny (biography on Henri El Salvador). but the people who fight for an ideal or the humanitarian. if Térésa Mother had not existed, if Jean Paul II had not existed the heroes are those which act, which helps, it is not inevitably a known character my father in my eyes was a hero here is the notion of the heroism clean to each individual those which fight and fight to live and to continue to live are of the heroes in my eyes l"heroism is often the fact of people little known and not involved opera singer, professor of song, is put in scene, originator of spectacles the Singer" Repetition for the spectacle on Edith Piaf. It large Maestro della young Musica and della Voce. trés already with the théatre of my birthplace (Toulon 83) I révais while looking at and applauding the operettas and the operas I awaited the artists at the exit, at one time or the public awaited the artist to make him sign the program and to chatter with lui.et each time I put the same question: "and now." and I set out again at home the eyes full of stars to have met the large artists of this time later, when I had my prices, I found myself with some of them that I admired but this time I etais partenaire.J' raised the heavy red curtain to them to see behind and today still "Speak to me d&rsquo.amour, my Pal the gipsy" Concert: "Beautiful Canto and Musique Crowned with the 19th S". Michael Tee with the Path of the Markets on April 18 Thank you infinitely for this nice comment and good road with you Aaaaaah, finally somebody who knows his traditional, that given pleasure. Thank you for this comment sympathetic nerve and cultural. Thank you for taking the time to check out my music. My new album, "Secret Journey", is out in record blinds worldwide. What has wonderful musical is this one too. This is has video of its 10 anniversary more than 10 years ago. Thank you Philippe, you are a true friend, and I will be very happy to see you around glass or of a good meal. Michael Tee with the Path of the Markets on April 18 Encore large a THANK YOU for your support the chanteur.Cela makes too a long time that one is vu.j' hopes that one will not wait too much. Thanks so much for the friend request. Pleasure to Be here amongst your circle of friends. For the latest information one David please subscribe to his blog: For information one MORNING SONG, click one these links: The. How to win photographs and updated contest details thank you of your compliments it is nice. Thank you very much of your comment, especially because it evokes "All up there". It is rare on Myspace to receive opinions on its work. Why your voice is not heard, on your page. Hey Philip-thanks for the well wishes. Michael Tee with the Path of the Markets on April 18. In fact I met your brother there is longtemp well, I was whereas a teenager lol then one was to find himself a few months ago then I do not have more news I hope that it is well passes to him my friendships, the world and small. North winds with one of these days perhaps. Michael Tee with the Path of the Markets on April 18 Thank you for your visit Philippe eta soon I espere.Bonne and happy year, with full with contrats.santé, happiness, large kisses of p' tite tete Philippe cuckoo good year with you. Thank you very much for your message A my turn I wish you all my wishes of happiness for this new year. I wish you as very full with beautiful things for this year which is announced as it is soft and full for you with success, than it brings to you health, happiness, love thank you with you, my turn I present to you my best wishes for this year 2008.une very good year at you. Happy New Year How Graphics Comments & Glitter Graphics for Myspace, Hi5, Orkut, Friendster. Full with good things for this new year. To when our return Galette of the Kings. I hope that you passed a good midnight supper and that people were charmed spectacle. I had come to bring your gift to you which the Christmas Dad had entrusted to me but I did not find anybody to open, snif. and with very quickly, since I have something which belongs to you ^^. Soul Power - Tribute to the 70s RnB Merci with you coming to listen to us. We wish you happy spend and an excellent year 2008 full with zic. Thank you for your so soft comment I wish you marvellous festivals of Christmas with all that you can wish thank you with you, I also wish you a Merry Christmas, friendship with you Merci with you and your encouragements, I advance and hope. Thousand kisses with you dear partner. Check out my album "Hurt By The Moon" At:. Thank you with you Philippe, much of happiness. I espere that you are well and that you passed a good week end. I wish you to spend a good night. The representations proceed very well and it may be that one begins again as from February 3:). I hope that all goes well for you. I espere that all occurs well for you. Me I am rather happy: the album left finally (in remote loading) and will be in the vats beautiful at the beginning of February universe by at home: -) Hello the Singer (so that' S you), thanks for the request. Check out my album "Hurt By The Moon" At:. Thank you Philippe for your invitation, very happy to meet you. Injury Shirley' S new album out now. Click one the album cover for information thank you with you when same, friendship with you. Thank you very much Philippe for your message and your compliments. They suit me sincerely right heart. It is not with you, I think, that I will learn that but when one likes, one does not count. And, I acknowledge, in art, I thus like I test all. I see that you also you one are impassioned touch-with-all. With the pleasure of meeting this mystèrieux Phantom of Verrières in live. PS: a small flat in your page if I can allow myself, an extract of you singing would be really brilliant good evening,il is necessary that I re-examine all that, friendship.

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Help to?hoisir me a chart of calls pr?y?Comment to use a chart of calls pr?y?VOX R?fund/Ajouter of the value?otre Information chart on the tariffs and expenses Which chart of calls pr?y?devrais I to choose. For the calls of short or long dur?au Canada and with?ats-Plain, the chart d&rsquo.appels pr?y? For do the calls fr?ents in Canada and?ats-Plain, use the chart d&rsquo.appels pr?y? Fresh Aucuns cach?&ndash. pay only for the utilis minutes?. Make your trunk calls with the chart d&rsquo.appels pr?y?quand want it to you, o?ous want it. The chart id?e for your d?acements. Carry out your calls overseas with the chart d&rsquo.appels pr?y?quand want it to you, o?ous want it. The chart id?e to speak?os overseas close. Use the chart d&rsquo.appels pr?y?pour to call your close relations overseas as often as you will d?rez it. The chart id?e to keep the contact. Have long discussions with your family and your friends overseas with the chart d&rsquo.appels pr?y? The chart id?e for your calls overseas of long hard? For do the intra-provincial calls, use the chart d&rsquo.appels pr?y? Fresh Aucuns cach?&ndash. pay only for the utilis minutes?. Why buy the charts of calls pr?y? VOX. To benefit from interurban tariffs tr?concurrentiels. Offer an average acc?au any. Is the interurban service s?ritaire, reliable and of qualit?ans aucuns fresh cach? VOX is?a height of your waitings. How to make to carry out a call by means of my NIP. To carry out a call, make the num? d&rsquo.acc?imprim? l&rsquo.endos of your chart and follow the vocal instructions: : 1 877 869-4540 (for the tariffs of VOX Minutes) or 1 877 869-4545 (for the tariffs of VOX More) For the calls?' int?or of Canada?ats-Plain and Cara?s, compose 1 + the code r?onal + the num? of t?phone. For do the international calls, compose 011 + the code of the country + the code of the city + the num? of t?phone. How to make to use this service by means of my t?phone cellular. To carry out a call, make the num? d&rsquo.acc?imprim? l&rsquo.endos of your chart and follow the vocal instructions: : 1 877 869-4540 (for the tariffs of VOX Minutes) or 1 877 869-4545 (for the tariffs of VOX More) For the calls?' int?or of Canada?ats-Plain and Cara?s, compose 1 + the code r?onal + the num? of t?phone. For do the international calls, compose 011 + the code of the country + the code of the city + the num? of t?phone. Your trunk calls will be factur?aux tariffs of services VOX. With regard to the time of antenna, your calls will be factur?par your supplier of cellular services. Can I use this chart for the international calls. Yes, you can carry out calls to?artir of Canada and thePlain ones towards more than 250 countries. Can I use this chart for t?phoner?a house to?artir of a site overseas. Can charts VOX?e utilis? to carry out calls to?artir of Canada and only thePlain ones. You cannot use these charts to carry out calls to?artir of a site overseas. How to make for conna?e the balance of my chart VOX. Once that you do enter your NIP the syst? t?phonic, the syst? you will inform of the balance of your chart. Also, before each call, the syst? you will warn of the number of minutes available for your call. You can also obtain the balance of your chart when you. Why I need a t?phone?lavier to carry out a call with my chart VOX. The use of the syst? of t?phone automatic to place a call a t?phone?lavier. requires, open a MonVOX session while entering your num? of user and your password, and then s?ctionnez the value?jouter. You will have to provide the information of your chart of cr?t for purposes of approval the funds will be ajout??otre account instantan?nt. If you do not have a MonVOX account yet, want to click on the bond. Which incidence that will have it on the scratch date of my account. The scratch dates vary according to the type of chart. . meter. sets out again?? when you add value?otre chart: VOX Combo Minutes/Plus, VOX More. How to obtain further information. You can communicate with an agent of our service?a customer?. We offer the support in any time and our repr?ntants will be able r?ndre?outes your questions concerning your chart VOX.: 1 866 761-8141 (for VOX Minutes) or 1 866 761-8139 (for VOX More) You have probl?s to?btenir. We offer the support in any time and our agents will be able r?ndre?outes your questions concerning your chart VOX.: 1 866 761-8141 (for VOX Minutes) or 1 866 761-8139 (for VOX More) Ultra VOX, VOX Overseas max, VOX Global How can I obtain information as for the expenses and the tariffs of the charts pr?y? VOX. Notes with the bottom of the pages, which d?tent by. *. contains also information as for the diff?nts fresh factur?et with the modalit?et conditions. Why I obtained less minutes than Pr?. Please consult the sections. Tariffs. produced diff?nts VOX to obtain the tariffs more r?nts of your favorite destinations. Which are the scratch dates of charts VOX. The dates d&rsquo.expiration vary according to the type of chart:. If I ach? will a chart pr?y?VOX today, the tariff be always the m? that that which is offered on the site. The tariffs are prone?hangement, and can?e modifi?de time?utre. The tariff in force will be always that pr?nt?n line. I still have a balance associ? my NIP, I can transf?r this balance towards another NIP. The service of transfer of balance allows you transf?r a r?duel amount of a NIP VOX Combo Minutes/Plus, VOX Plus or VOX Minutes?n other. Make simply one of the following num?s, and follow the vocal instructions for the service of transfer of balance (option 1 of the menu): VOX Combo Minutes/Plus: 1 866 761-8141 (for VOX Minutes) or 1 866 761-8139 (for VOX More) VOX Minutes or VOX More r?li? : 1 877 819-8862. Unfortunately this service is not available for other charts VOX. Can I use this chart for t?phoner with the service d&rsquo.assistance directory (411). This type of service n&rsquo.est not available. Can I use this chart to call another num? without expenses. Fresh Aucuns are not associ??es num?s. Can I use this chart to call the t?phonist (0). This type of service n&rsquo.est not available.

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fluidly, liquid, fluxional wear and tear, scuff, wearout illness, malady, diesease, sickness, disease insecure, unsure, unstably, aleatory, uncertain send, to broadcast, to transmit, broadcast, emit manly, viril, unfeminine, evil, locate, set, situated masculine, put, install, place set, install, place, locate, situated, put adopt, taking over, assume, take over adopt, taking over, assume, take over precisely, carefully, exactly, precise, exact abominable, undelightful, awful, horrible, rotten natural, artless, unstudied, elemental, of course in two Time, in two course, at two dates henchman, hanger, devotee, trailer, follower licence, approval, permission, permit, approbation swimming, swim, floatage, flotage, float slopingly, slanting, sloped, oblique, transversal askance, lopsided, wry, sloping, slanting, askew oafishness, stupidity, stupid thing, asininely busy, engaged, occupies, taken, occupied beyond, across, on the other side decided, pit in order, organize, rows, arrange ordinary, common, ornery, usual, usually slip of paper, notes, slip, schedele recieve, maintain, get, recd, receive, obtain carpark, parking lot, car Park upright, even, still, exactly, straight afraid, anguish, fear, fright, distress mad, screwy, crazy, loony, madly pain, ache, sorrow, achiness, grief retired person, old age pensioner hence, serious therefore, therefor, thats why emend, improve, impove, amend, ameliorate, correct judicious, sharp witted, subtle, sly, hard, heavy, weighty, serious, difficult hairstyle, hairdo, haistyle, headdress, hair style portion, ration, slice, play, piece townplan, City map, map of the City whacked, socked, slugged, slatted, flicked, tolled slat, slap, strike, beat, sock, slug, hit slothful, rottenly, idle, lazy, sluggard loosely, ungirt, limber, unformal, loose, fluffily function, position, job, attitude, emplacement guardedness, take beloveds, circumspectness, caution beg, request, ask request, ask beginning, outset, origin, start, entrie, top 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frankly, openly, unharbored, forthright to detect, reveal, undeck, detect, discover label, notice, sign, tag, plate, signpost excuse, excuse me, apology, sorry, alibi dress, commit oneself, join, set up, rest, buddle sound, according to, noisy, unquiet, loud, aloud wheelchair, roll chair, wheel chair haggard, rampant, frantically, tomboyish, tigerish unit, easy, simply, simpleminded, simple, rustic one way, one way street dissociated, separated, unlink, disrupt, disjoin articulative, yeah, unobscured, clearly, clear handle, servants, operated, attend, attend to safely, certain, assuredly, sure, unmistaken, safe wife, mrs., woman, Madam, Mrs, tradesman mademoiselle, Young lady, Miss, Miss. characters, char, chars, mark, signs, sign simultaneous, simultaneously, at the same Time only, solitary, discreet, singly, single, unique jingle, tringing, ring, tinkle, ting hyper, over, of, via, across, above, on outdated, overhauls, dated, overtakes, out of dates beneath, among, under, lower, 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(WO/2003/099991) ADMINISTRATION Of A GENE GLP-1 POUR TRAITEMENT DU DIABETES DE TYPE 2 (WO/2003/099991) ADMINISTRATION Of A GENE GLP-1 POUR TRAITEMENT DU DIABETES DE TYPE 2. Ils contiennent de ce fait DES inexactitudes vis?is DES documents originaux ET n' ont pas de valeur l?le. GLP-1 Gene Delivery for the Treatment of Type 2 Diabetes This invention relates to compositions and methods of use thereof to normalize the blood glucoses levels of patients with type 2 diabetes. More particularly, the invention relates to to composition and method for the delivery of the GLP-1 gene, both in vitro and in alive, for the. It relates particularly to to plasmid comprising to chicken (3 actin promoter and enhancer. to modified GLP-1 (7-37) situated, which is constructed and delivered into to cell for the expression of active GLP-1. invention also encompasses transfecting compositions comprising to complex of to plasmid and poly (ethylenimine) (PEI) (for in vitro gene delivery) or SATISFIED (for in alive gene delivery). Type 2 diabetes is characterized by hyperglycemia, insulin resistance, absolute or relative insulin deficiency, hyperglucagonemia, and increased hepatic glucoses production. Although many treatment trials for type 2 diabetes have been held, to there is still not definitive. Insulin secretion is modulated by incretin hormones which to are produced by the intestinal enteroendocrine cells and constitute one arm of the enteroinsular axis. There to are two major incretins. One is glucoses-dependent insulinotrophic polypepetide (JEEP) and the other is glucagon like peptide-1 (GLP-1). These two incretin hormones account for 20% and 80% respectively, of the intestinal incretin effect. GLP-1, tends to lose its actions in patients with type 2 diabetes. Orskov C, Holst JJ, Ebert R, Creutzfeldt W: Preserved incretin activity of glucagons-like peptide 1 [ 7-36 amide ] but not of synthetic human gastric inhibitory polypeptide in patients with. GLP-1 was recently used for the. See US Patent Nos 5,614, 492. 5,545, 618 and 6,048, 724 which to are. GLP-1, produced by intestinal L-cells, stimulates glucoses induced insulin secretion and. Active GLP-1 has two forms, GLP-1 (7-36) amide and GLP-1 (7- 37), that to are products of posttranslational processing of proglucagon in mammalian intestinal. The active forms of GLP-1 to are degradable in the plasma by the action of dipeptidyl. During degradation, the active form of GLP1 (7-36 or 7-37) loses its N-terminal loves acid residues and results in an inactive form of GLP-1 (9-36 amide). active forms of GLP-1 have very short plasma half lives (about 5 minutes) and metabolic. to: Endocr Rev 16: 390-410,1995 There have been several studies on administration of GLP-1 to type 2 diabetic patients which have shown that GLP-1 effectively reduces hyperglycemia in type 2 diabetic patients. However, it is very difficult to consistently deliver the active form of GLP-1 because of its short half-life. Even when using the long acting form of exendin-4, twice daily administration is required to maintain to normal glucoses level. Doyle ME, Betkey JA, Holloway HW, Spencer RG, Greig NH, Egan JM: Exendin-4 decelerates food intake, weight gain, and fat deposition in Zucker rats. Gene therapy is generally considered as to promising approach, not only for the treatment of diseases with genetic defects, but also in the development of strategies for treatment and prevention of chronic diseases such as cancer, cardiovascular disease and diabetes. nucleic acids, as well as other polyanionic substances to are rapidly degraded by nucleases and exhibit poor cellular uptake when delivered in aqueous solutions. methods for delivery of nucleic acids in tissue cultures cells in the mid 1950'.s, steady progress has been made towards improving delivery of functional DNA, RNA, and antisense. The gene carriers used I know to make includes viral systems (retroviruses, adenoviruses, adeno- associated viruses, or herpes simplex viruses) or nonviral systems (liposomes, polymers, peptides, calcium phosphate precipitation and electroporation). Viral vectors have been shown to have high transfection efficiency when compared to non-viral vectors, but two to several drawbacks, such as targeting only dividing cells, random DNA insertion, their low capacity for carrying large sized therapeutic genes, risk of replication, and possible immune host reaction, their use in alive is severely limited. An ideal transfection reagent should exhibit to high level of transfection activity without the need for any mechanical or physical manipulation of cells or tissues. non-toxic, or minimally toxic, at the effective dose avoid any long term adverse side effects on the treated cells. When gene carriers to are used for delivery of nucleic acids in alive, it is essential that the gene carriers themselves be nontoxic and that they degrade into non-toxic products. To minimize the toxicity of the intact gene carrier and its degradation products, the design of gene carriers needs to be based on naturally occurring. As compared to viral gene carriers, to there to are several advantages to the use of non-viral based gene therapies, including their relative safety and low cost of manufacture. polymeric materials currently being investigated for use as gene carriers, of which poly-L-lysine (PLL) is the most popular, but few of them to are biodegradable. to are known to be toxic and the PLL backbone is barely degraded under physiological conditions. It remains in cells and tissues and causes an undesirably high toxicity. cationic polymers, combined PLL/DNA complexes have drawbacks including precipitation as insoluble particles and the tendency to into larger complexes under physiological conditions. In view of the foregoing, to there is to need for the development of to composition and to gene therapy method for the treatment diabetes wherein the gene carrier is soluble and biodegradable, meaning that the non-viral polymer gene carrier can break down or degrade within the body to non-toxic components after the genes have been delivered. The present invention provides to composition and to method for delivery of the GLP-1 gene, both in vitro and in alive, for the treatment of type 2 diabetes. actin promoter and enhancer. to modified GLP-1 (7 -. transfecting compositions comprising to complex of to plasmid containing to modified GLP-1 (7- and poly (ethylenimine) (PEI) (for in vitro gene delivery) or SATISFIED (for in alive gene delivery), at to proper weight ratio. Particularly, the weight ratio of DNA to the cationic biodegradable gene carrier is preferably within to range 1: 0, DNA/PAGA, the ratio is preferably with the range of 1: 0.46 and for DNA/PEI, the ratio is preferably with the range of 1: 0, This invention also provides for to method of transfecting to cell with the (a) providing to composition comprising effective amounts of complexes of to plasmid containing and to polycationic gene carrier at to proper weight (b) contacting the cell with an effective amount of the composition such that the cell internalizes culturing the cell with the internalized DNA under conditions favorable for the growth. This invention further provides for to method of normalizing the blood glucoses levels of an animal with type 2 diabetes, comprising the steps of: (a) providing to composition comprising effective amounts of complexes of to plasmid containing (b) administering to the animal to be treated an effective amount of the composition such that. Additional features and advantages of the invention will be apparent from the detailed description which follows, taken in conjunction with the accompanying drawings, which together illustrated, by way of example, features of the invention. sequence and to furin cleavage situated. 2 shows the RT-PCR assay of the production of. 3 shows the insulin secretion of Co-cultured islets with HepG2 cells transfected by the. illustrates the therapeutic effect of the transfection composition containing complexes in Zucker Diabetic Fatty (ZDF) rats. Before the present composition and method for treatment described, it is to be understood that this invention is not limited to the particular configurations, process steps, and materials disclosed herein as such configurations, process steps, and materials. It is also to be understood that the terminology employed herein is used for the purpose of describing particular embodiments only and is not intended to be limiting since the scope of the present invention will be limited only by the appended claims and. It must be noted that, as used in this specification and the appended claims, the singular plural referents unless the context clearly dictates otherwise. In describing and claiming the present invention, the following terminology will be used in accordance with the definitions set forth below. mean transport of nucleic acids from the environment external to to cell to the internal cellular environment, with particular reference to the cytoplasm. Without being bound by any particular theory, it is understood that nucleic acids may be delivered to cells either after being encapsulated within or adhering to one or more cationic lipid/nucleic acid complexes or entrained therewith. deliver to nucleic acid to to cell nucleus. Nucleic acids includes both DNA and RNA as well as. Such nucleic acids includes as well as protein producing nucleotides, on and off and installments regulatory nucleotides that control protein, peptide, and nucleic. In particular, but nonlimiting, they can be genomic DNA, hybrid sequences or synthetic or seeds-synthetic sequences, and of natural or. In addition, the nucleic acid can be variable in size, ranging from. They may be obtained by any technique known to to person. As used herein, the term"biodegradable"or"biodegradation"is defined as the conversion of materials into less complex intermediates or end products by solubilization hydrolysis, or by the action of biologically formed entities which can be enzymes and other products of the. As used herein, "bioactive effective amount"means an amount of to nucleic acid or agent that is sufficient to provide the desired local or systemic effect and performance at to reasonable risk/benefit ratio as would attend any medical treatment. As used herein, "administering", and similar terms means delivering the composition to the individual being treated such that the composition is capable of being circulated systemically to to where the composition binds to to target cell and is taken up by. Thus, the composition is preferably administered to the individual systemically, typically by subcutaneous, intramuscular, intravenous, or intraperitoneal administration. Injectables for such use can be prepared in conventional forms, either as to liquid solution or suspension, that is suitable for preparation as to solution or suspension in to liquid prior to. Suitable excipients that can be used for administration includes, for example, water, goes up some, dextrose, glycerol, ethanol, and the like. and if desired, minor amounts of auxiliary substances such as wetting or emulsifying agents, buffers, and the like. According to the invention to there is provided to plasmid suitable for expression, consisting essentially of an expression facilitating sequence derived from chicken actin promoter and enhancer. an expression sequence comprising ATG (start codon) followed by to sequence (CGTCAACGTCGT) coding for to furin cleavage situated (FCT) and to sequence coding for the active form of GLP-1 that to are operably linked to said expression facilitating. Optionally, the plasmid may contain to non-mammalian origin of replication. and to sequence operably encoding to selectable marker. One important aspect of the present invention relates to the addition of the furin cleavage situated between the start codon and the GLP-1 (7-37) gene. It is common knowledge that all gene expression requires to start codon which encodes methionine at the N terminial of to protein. are products of post-translation processes in alive terminal loves acid of GLP-1 (methionine) produced from the plamid is not cleaved, it will block the activity of the first two loves acids of which to are the receptor binding domain, active lose its biological activity completely. plasmid that can express high levels of forms of GLP-1, to furin cleavage situated (FCT) is introduced between the start codon and the GLP-1 (7-37) sequence. transmembrane protein composed of to signal peptide, to propeptide terminating in an endoproteolytic cleaveage situated comprised of to cluster of basic residues, to subtilisin-like catalytic domain, middle domain, and to cysteine-rich domain followed by C-terminal transmembrane. produced from the plasmid constructs, according to the present invention, to are secreted from the cells, the N terminal methionine-FCT of the expression product will be cleaved by the furin in the Golgi apparatus of the cells. benefit of choosing FCT is that furin is widely distributed in most cell types, I know that the plasmid constructs of the present invention can efficiently express active. Although the plasmid constructs of the present invention to are disclosed for in alive gene delivery of active GLP-1, the usage of FCT is applicable to whose gene delivery of any peptide activity requires cleavage of the N-terminal methionine of the expression product and they intended to be within the scope of the invention. In one embodiment of the present invention, the plasmid may possess the functional characteristics of the plasmid comprising to nucleotide sequence represented by SEQ ID NOT: 1, One example of the plasmid of the present invention has to nucleotide sequence represented by. In another embodiment, the plasmid consisting essentially of an expression facilitating promoter and enhancer. an expression sequence comprising ATG (start codon) followed by to sequence (CGTCAACGTCGT) coding for to furin cleavage situated (FCT) and to sequence coding for the active form of GLP-1 thereof that to are operably linked to said expression facilitating sequence. In another embodiment, the invention provides to host cell transformed by any of the. In still another embodiment, the invention provides to method for producing any of the above plasmids, comprising the steps of: growing bacterial cells containing the plasmid. and recovering the plasmid from the bacterial cells. In an additional embodiment, the invention provides to pharmaceutical composition comprising the plasmid in combination with to pharmaceutically acceptable vehicle. plasmid may be complexed to to biodegradable cationic polymeric gene carrier The invention also provides to eukaryotic expression vector for the expression of to DNA sequence in to human tissue, consisting essentially of: an expression facilitating sequence derived from chicken P-actin promoter and enhancer. an expression sequence comprising ATG (start codon) followed by to sequence (CGTCAACGTCGT) coding for to furin cleavage situated (FCT) and to sequence coding for the active form of to bioactive peptide that to are operably linked to said expression facilitating sequence, and to sequence containing to transcriptional termination and to polyadenylation signal. and to selectable marker. wherein the vector is capable of replicating in. In another embodiment, the invention provides to method of gene therapy, the improvement comprising administering any of the above plasmids directly into to cell resulting in the local secretion of active GLP-1, active GLP-1 derivatives or other peptides. It will be readily apparent to one skilled in the art that various substitutions and modifications may be made to the invention disclosed herein without departing from the scope. This approach differs from previous methods in which cells are collected, propagated in vitro, modified and selected and then king-injected in alive includes, 1) the need to establish to cell linens from each experimental subject to avoid tissue rejection, 2) concerns about alteration of the phenotype of cells propagated in tissue cultures, 3) outgrowth of aberrant transformed cells, and 4) the Time and effort required. method, genes to are directly transferred into an animal with type-2 diabetes to where the cells take. DNA expression is facilitated by introducing the DNA complexed with to cationic biodegradable gene carrier. The those gene carrier facilitates entry of the DNA into cells thus providing for intracellular access to the DNA/gene carrier complex. DNA to patients in to drug-like manner is thus facilitated. Classical pharmacological studies of drug distribution, half life, metabolism, and excretion to are not entirely relevant to in alive gene injection and expression. the plasmid and detection of the gene product (GLP-1) to are relevant to the development of this. Therefore, as part of the measurement of the efficacy of this study, successful gene transfer and expression is evaluated by molecular and immunological analyses. parameters to are measured to evaluate the transfection and expression of active GLP-1 (7-37): 1) which encodes the Met-FCS-GLP-1 from the plasmid is assessed by RT-PCR amplification of cells obtained after transfection, 2) secretion of active cells transfected with the plasmid in vitro is measured by enzyme linked immunosorbent assay (ELISA), 3) measurement of insulin secretion of Co-cultured islets with GLP-1 plamid transfected cells, and 4) serum glucoses levels to are measured pre-treatment and after the start of. The plasmid suitable for GLP-1 expression is to eukaryotic expression vector that codes. To process for the production of this plasmid has been developed. The process is saleable and is to combination of highly reproducible unit operations (fermentation, cell lysis, precipitation, size exclusion chromatography, formulation. The advantages over existing methods includes scalability, improved plasmid purity and the elimination of undesirable process additives such as toxic solvents and animal derived. One skilled in the art will readily appreciate that the is representative of to preferred embodiment that is exemplary and not intended to be to limitation Any eukaryotic expression vector that is adapted to carry out the objectives and obtain the ends and advantages mentioned as well as those inherent herein is encompassed within the. Accordingly, the plasmids of the present invention to are assembled out of components comprising to gene encoding an active peptide such as GLP-1, origins, promoters, polyadenylation signals, and furin cleavage sites (FCS). plasmid of the present invention is diagrammed in FIG. nucleotide sequence of the coding strand of the plasmid is given as SEQ ID NOT: 2. high copy number plasmid that was constructed using isolated segments of synthesized GLP-1 (3-37, using standard molecular genetic techniques and commercially available enzymes. The backbone plasmid DNA is derived from pCI (Promega Inc. vector widely used in molecular biology laboratories. Development of to safe and efficient gene delivery carrier is an important factor to. Preferably, the cationic biodegradable polymeric gene carrier used in the present invention can spontaneously form discreet nanometer-sized particles with to nucleic acid, which can promote more efficient gene transfection into mammalian cells and. The biodegradable gene carrier, such as SATISFIED, is readily susceptible to metabolic degradation after incorporation into animal cells. polymeric gene carrier can form an aqueous micellar solution which is particularly useful for the. The present invention further provides transfection formulations, comprising to biodegradable cationic polymeric gene carrier complexed with an expressive gene vector carrying the GLP-1 gene, in the proper weight ratios (positive charge of the gene carrier/negative charge of the nucleic acid), that is optimally effective for both in alive and in vitro transfection. Preferably, the biodegradable SATISFIED gene carrier is and the gene vector is. the weight ratio of DNA to the cationic biodegradable gene carrier is preferably within to range. For DNA/PAGA, the ratio is preferably with the range of 1: 0. for DNA/PEI, the ratio is preferably with the range of 1: 0, The gene carrier of the present invention can also be conjugated, either directly or via spacer. The targeting ligands conjugated to the penetrated gene carrier direct the gene carrier-nucleic acid/drug complex to bind to specific target cells and into such. The targeting ligand can also be an intracellular targeting element, enabling the transfer of the nucleic acid/drug to be guided towards certain favored cellular compartments (mitochondria. An advantage of the present invention is that it provides an efficient transfecting composition for delivery of to GLP-1 gene into to cell wherein the particle size and charge density. Control of particle size is crucial for optimization of to gene delivery system because the particle size often governs the transfection efficiency, cytotoxicity, and. In general, order to enable its effective penetration into tissue, the size of to gene delivery particle should not exceed the size of to virus. of the invention, the particle sizes will range from about 80 to 200 nm depending on the cationic copolymer composition and the mixing ratios of the components. nanospheres, and microspheres of different sizes, when injected, accumulated in different organs of the body depending on the size of the particles injected. administration, particles of less than 150 nm diameter can pass through the sinusoidal fenestrations of the liver endothelium and become localized in the spleen, and bone marrow. Intravenous, intra-arterial, or intraperitoneal injection of particles approximately 0, diameter leads to rapid clearance of the particles from the blood stream by macrophages of the. It is believed that the presently claimed composition is effective in delivering the GLP-1. The cationic polymeric gene carrier may further comprises to targeting moiety is selected from the group consisting of transferring, asialoglycoprotein, antibodies, antibody fragments, low density lipoproteins, interleukins, GM-CSF, G-CSF, M-CSF, stem cell factors, erythropoietin, epidermal growth factor thrombomodulin, fusogenic agents such as polymixin agents, and nucleus localization signals (NLS). cells can be carried out by matching to cell having to selected receptor thereof with to selected. Alive therefore The present invention provides methods for treating type 2 diabetes by in delivery of an expressive gene vector which provides to desirable amount protein during to sustained period of Time. The biodegradable transfecting composition of the present invention, as described herein, exhibits improved cellular binding and uptake characteristics toward the. As such, the present invention overcomes the problems as set forth above. example, the biodegradable gene carrier of the present invention, such as SATISFIED, is easily hydrolyzed or degraded into low molecular weight components which will be easily eliminated. In addition, the degradation products to are small, non-toxic molecules that to are subject to renal excretion and to are inert during the period required for gene expression. Moreover, cells transfected with the plasmid of the present invention, namely, can produces the most potent form of natural. There to are two characteristics in the plasmid constructs of the present invention that afford the capability of producing the most potent form of natural GLP-1 peptide. First of all, to there is to furin cleavage situated between the start codon and the GLP-1 gene, thus furin cleaves the first loves acid, methionine, which is encoded. Therefore, the cleavage of active methionine preserved the function of the next two receptor binding domain and which is essential for. actin promotor of the CMV promotor provides the plamids the capability of expressing higher amounts of the GLP-peptide in cells because chicken (3 actin promotor is to more potent promotor than the CMV promotor. More The following examples will enable those skilled in the art to clearly understand how to practice the present invention. It is to be understood that, while the invention has been described in conjunction with the preferred specific embodiments thereof, that which follows is intended to illustrated and not limit the scope of the invention. Other aspects of the invention will be apparent to those skilled in the art to which the invention pertains. This example illustrates the construction of the plasmid which expresses. expression was driven by chicken (3 actin promoter and enhancer. (7-37) gene was synthesized using to DNA synthesizer. The start codon encodes methionine. the N-terminal loves acid of the produced is methionine, it will not be an active form, because the first two loves acids of to are the receptor binding domain. recognition situated (ArgGlyArgArg: CGTCAACGTCGT) was inserted into the synthesized GLP-1 (7-37) gene just after the start codon. When the produced GLP-1 is secreted from the cell, the methionine is cleaved by the furin in the Golgi apparatus. To replace the promoter part (chicken actin promoter and CMV promoter), chicken promoter and enhancer to were isolated from. The CMV promoter of pCI (Promega Inc. Both digested DNA fragments to were confirmed by their size using to gel. And then the DNA fragments in agarose gels to were extracted using Gel Extraction.) The gel extraction was performed following their prescribed procedures two extracted fragments to were ligated using T4 DNA ligsae (Promega Inc. hours, and we named our final plasmid. The synthesized GLP-1 (7-37) was treated with Kpnl and XbaI and inserted into the KpnI and XbaI sites. ligation methods to are same as described above. JM109 (Promega) and purified by an alkaline lysis method using the Maxi Prep Kit (Qiagen). The purity of the plasmid was confirmed by absorbance at 260 nm and 280 nm and the quantity. The constructed plasmid was confirmed by DNA. SATISFIED Synthesis of and formation of PAGA/DNA complex This example illustrates the preparation of to gene delivery composition, according to the. SATISFIED was synthesized as previously described in US Patent Not Briefly, CBZ-L-oxylysine was synthesized from CBZ-L-lysine. CBZ-L-oxylysine was polymerized by to melting condensation reaction at. The polymer was dissolved with chloroform and precipitated. The dried polymer was dissolved with DMF containing to palladium activated. 85% formic acid was added as to proton donor. temperatures, the polymer solution was isolated from the catalyst by addition polymer was precipitated with acetone. SATISFIED was stored at-70°.C until use. The DNA/carrier complexes to were prepared by self assembly. Ten times diluted carrier solution was slowly added into the prepared DNA plasmid and left for 30 minutes to allow for formation of complexes. Stable complexes to were formed with SATISFIED and the aqueous plasmid DNA solution based on the fact that not precipitation or aggregation was observed at wide concentration ranges of the. Complex formation of the plasmid DNA and the cationic copolymer was tested by agarose gels. The results show that complete neutralization was achieved from the weight ratio of DNA to the cationic polymer is within to range of 1: 0, This example illustrates the expression of. analyze the expression of the gene, RT-PCR was carried out on the was transfected into HepG2 cells used to evaluate the expression of GLP-1 HepG2 cells to were maintained in MEM supplemented with 10% FBS in to 5% C02 incubator. For the transfection studies, the cells to were seeded at to density of 2, plates and incubated for 24 hours before the addition. complexes to were prepared by mixing plasmid and PEI at an N/P ratio of 1: 5 (the.676) in serum free MEM medium and then incubated for 30 minutes at. The cells to were washed twice with PBS, and then 2 mililiter of fresh serum-free. complex was added to each well. After 4 hours, the transfection mixtures to were removed and 2 mililiter of fresh MEM containing FBS was added. Medium We collected the that was treated with dipeptidyl. Then to GLP-1 assay (Linco Inc.) was carried out following the suppliers The transfected cells to were washed twice with PBS, and total RNA was harvested by acid- guanidium thiocyanate-phenol-chloroform extraction, using RNAwiz (Ambion, Austin, TX). The concentration of RNA was measured by the absorbance at 260 was confirmed by formaldehyde-formamide denatured agarose gels electrophoresis. micrograms of total RNAs to were hybridized to the backward primer and reverse transcribed using AMV reverse transcriptase (Promega, Madion, WI). to were amplified by polymerase chain reaction (PCR), using Taq polymerase (Promega, Madison. The sequences of the specific oligonucleotide primers to were as follows: primer, 5'.-CAGAAGTTGGTCGTGAGGCA-3'. GLP-1 backward primer, 5'. -. The PCR reaction was performed in 25 cycles at. The PCR products to were analyzed in 2% agrose gels electrophoresis. The transfected HepG2 cells expressed. Therefore, this result suggests that the detected. Also, the expression level can. ELISA detection of GLP-1 in transfected HepG2 cells This example illustrates the expression of GLP-1 in transfected HepG2 cells. expression of the active GLP-1 peptide, ELISA was carried out on the that was transfected into HepG2 cells used to evaluate the production of active GLP-1. HepG2 cells to were cultured and transfected with the plamid of the present invention as. In order to confirm that the expressed purpose active GLP-1 should be secreted and for therapeutic, active ELISA assays for the form of hours after transfection, using the active GLP-1 ELISA kid (Linco, Inc). 2B, the active form of GLP-1 was detected in the cell cultures medium by an ELISA assay, suggesting that the expressed GLP-1 by ppGLP1 was secreted into the cell cultures medium production of the transfected HepG2 cells was 24, pß.GLP1 transfected ElepG2 cells The example illustrates the effect of the secreted. Islets of Langerhans to were isolated from Sprague-Dowley badly rat pancreas, by to collagenase digestion technique and discontinuous Ficoll density gradient centrifugation. Briefly, the pancreas was removed after swelling caused by collagenase solution injection (Type V, Sigma, 10-15 ml/pancreas, lmg/ml in HBSS) through the common bile duct, and incubated. The digested fragments of the pancreas to were collected and washed with. Finally, the acinar cells and islets to were separated by Ficoll density gradient. The islet rich layer was collected and washed with HBSS, and the islets to were suspended in RPMI-1640 medium with 10% FBS and. For the Co-cultures study, the isolated islets to were sub-cultured for. For transfection, HepG2 cells cultured in MEM supplemented with 10 % FBS to were used. The cells to were seeded at to density. hours incubation, the cultures free medium was changed to FBS fresh medium and loaded with complexes (PEI/DNA N/P ratio 5 the weight ration of DNA/PEI is 1: 0, DNA/well) followed by 4 hours incubation at. medium was changed to fresh MEM with 10% FBS and the cells to were incubated for another 48 hours before the Co-cultures study at. The Co-cultures study was performed with isolated islets and. 30 islets with to small size distribution (mean size of transfected HepG2 cells cultures system. The islets and HepG2 cells to were separated by to physical barrier (cell cultures insert). Then, the cultures medium was changed to.5 mililiter) supplemented with 10% FBS with basal and high glucoses. After 4 h Co-incubation, the insulin and GLP-1 content in the medium was measured by RIVER and ELISA, respectively. The transfected HepG2 cells of each well produced from 3, was not enhancement of insulin secretion under to low glucoses concentration (50 mg/dL). However, to remarkable increment of insulin secretion occurred under high glucoses stimulated the secretion of insulin under high glucoses conditions but did not stimulate insulin secretion under low glucoses conditions. complex was delivered into type 2 diabetic animals in. The type 2 diabetic animal was ZDF (Zucker Diabetic Fatty) rats. homozygote leptin receptor mutations, I know they seem to have similar manifestations of human. The DNA/carrier complexes to were prepared by self assembly. SATISFIED Before addition of into the DNA, the DNA was diluted in. Ten times diluted carrier solution was slowly added into the prepared DNA plasmid and left for 30 minutes to allow for formation of complexes. (DNA/PAGA weight ratio was 1: 1.23) The total volume of DNA/PAGA. Before injection, the rats to were incised in their right side neck areas under anesthesia to expose. complex was injected through the. One rat was injected with of plasmid and another was injected with. The blood glucoses levels to were checked every other day using to portable glucometer. The blood glucoses levels to were normalized 7 days after injection of the. Also, normal 150 glucoses levels to were sustained for 4 weeks. levels of the treatment group to were maintained at less than mg/dL. polymeric gene carrier complex to patients with type 2, complex will be delivered into type 2 diabetic patients. prepared as in Example 6 will be injected into the peripheral. Each patient will be injected with 20 mg of plasmid DNA. every day for four days.6 mg in 200 mililiter, 5% glucoses solution) will be injected to control group. treatment group will be composed of twenty type 2 diabetics, respectively. will be collected every three days for plasma insulin and. concentration will be measured four times every day. It is expected that among the patient who complex, the blood five glucoses levels should be normalized about to ten days after injection. level is expected to be sustained for at least weeks. Thus, among the various embodiments taught to there has been disclosed to composition and gene, both in vitro and in alive, for the treatment of type 2. It will be readily apparent to those skilled in the art that various changes and modifications of an obvious natures may be made without departing from the spirit of the invention, and all such changes and modifications to are considered to fall within the scope of the invention as defined by the appended claims. Subscribe to receive e-mails of news and updates on WIPO' s activities regarding patents and the PCT